2023
Junod S., Tingey M., Kelich J., Goryaynov A., Herbine K., Yang W.* (2023) Dynamics of nuclear export of pre-ribosomal subunits revealed by high-speed single-molecule microscopy in live cells. iScience 26, 8, 107445, https://doi.org/10.1016/j.isci.2023.107445.
Yu W., Rush C., Tingey M., Junod S., and Yang W.* (2023) Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics. Chem. Biomed. Imaging 1, 4, 356–371.
Ruba A., Tingey M., Luo W., Yu J., Evangelou A., Higgins R., Khim S., Yang W.* (2023) The Ciliary Lumen Accommodates Passive Diffusion and Vesicle-Assisted Trafficking in Cytoplasmic-Ciliary Transport. Molecular Biology of the Cell 34(6):ar59. doi: 10.1091/mbc.E22-10-0452. Epub 2023 Mar 1.
Junod S., Tingey M., Rush C., Alkurdi A., Bajoria K., Yang W.* (2023) Obtaining 3D super-resolution images by utilizing rotationally symmetric structures and 2D-to-3D transformation. Computational and Structural Biotechnology Journal 21:1424-1432.
Tingey M., Ruba A., Yang W.* (2023) High‐speed Super‐Resolution SPEED Microscopy to Study Primary Cilium Signaling in vivo. Methods in Cell Biology ISSN 0091-679X, https://doi.org/10.1016/bs.mcb.2022.12.016
Rush C, Jiang Z., Tingey M., Feng F., Yang W.* (2023) Unveiling the complexity: assessing models describing the structure and function of the nuclear pore complex. Frontiers in Cell and Development Biology. 11, https://doi.org/10.3389/fcell.2023.1245939.
Tingey M., Junod S., Rush C., and Yang W.* (2023) Protocol for live-cell super-resolution imaging of transport of pre-ribosomal subunits through the nuclear pore complex. STAR Protocols. DOI: 10.1016/j.xpro.2023.102790.
2022
Tingey M., Li Y., Yang W.* (2022) Selective Degradation and Quantification of Nucleoporins in the Nuclear Pore by Auxin-inducible Degrons and Single-molecule Microscopy, Current Protocols, 2, e520. doi: 10.1002/cpz1.520 .
Islam, M.S., Junod, S.L., Zhang S., Buuh Z., Guan Y., Zhao M., Kaneria K., Kafley P., Cohen C., Maloney R., Lyu Z., Voelz V., Yang W. & Wang R* (2022) Unprotected Peptide Macrocyclization and Stapling via a Fluorine-thiol Displacement Reaction. Nature Communications 13, 350.
Tingey M., Schnell S.+, Yu W.+, Saredy J., Junod S., Patel D., Alkurdi A., Yang W.* (2022) Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA, Cells, 11, 3079.
Tingey M., Li Y., Yu W., Young A., Yang W.* (2022) Spelling out the roles of individual nucleoporins in nuclear export of mRNA, Nucleus, 13:1, 170-193.
Tingey M. & Yang W.* (2022) Unraveling docking and initiation of mRNA export through the nuclear pore complex. BioEssay 44(8):e2200027.
Schnell S.J., Tingey M., Yang W.* (2022) SPEED microscopy: high-speed single-molecule tracking and mapping of nucleocytoplasmic transport. Methods in Molecular Biology 2502. Humana, New York, NY.
2021
Li Y., Aksenova V, Tingey M., Yu J., Ma P., Arnaoutov A., Dasso M. and Yang W.* (2021) Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore. Proc. Natl. Acad. Sci. USA, 118, (37) e2015621118.
Li Y., Tingey M., Ruba A. and Yang W.* (2021) High-speed super-resolution imaging via SPEED microscopy and 2D to 3D transformation. Nature Protocols, 16, 532-560.
Dixon C. R., Malik P., Heras J., Saiz-Ros N., Alves F., Tingey M., Gaunt E., Richardson A. C., Kelly D., Goldberg M., Towers G., Yang W., Rappsilber J., Digard P., Schirmer E. C. (2021) STING Nuclear Partners Contribute to Innate Immune Signalling Responses. iScience, S2589-0042(21)01023-3.
Tingey M., Li Y., Yang W.* (2021) Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells, STAR Protocols 2, 100490, 2021.
Junod S., Saredy J. and Yang W.* (2021) Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy, Viruses 13, 167.
Schnell S.J., Tingey M., and Yang W.* (2021) SPEED microscopy: high-speed single-molecule tracking and mapping of nucleocytoplasmic transport. Methods in Molecular Biology (in press).
2020
Li Y., Tingey M., Ruba A. and Yang W.* (2020) High-speed super-resolution imaging via SPEED microscopy and 2D to 3D transformation. Nature Protocols https://doi.org/10.1038/s41596-020-00440-x.
Dixon C. R., P. Malik., J. I. de las Heras., N. Saiz-Ros., F. de Lima Alves., M. Tingey., E. R. Gaunt., Richardson C. A., Kelly D. A., Goldberg M. W., Towers., Yang W., Rappsilber J., Digard P., Schirmer E. C. (2020) STING Nuclear Partners Contribute to Innate Immune Signalling Responses. bioRxiv https://doi.org/10.1101/2020.12.21.423744. submitted to Nature Communications
Mudumbi K., Czapiewski R., Ruba A., Junod S., Li Y., Luo W., Ngo C., Ospina V., Schirmer E. and Yang W.* (2020) Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores. Nature Communications 11:2184. https://doi.org/10.1038/s41467-020-16033-x.
Junod S., Kelich J., Ma J. and Yang W.* (2020) Nucleocytoplasmic transport of intrinsically disordered proteins studied by high-speed super-resolution microscopy. Protein Science 29: 1459-1472. https://doi.org/10.1002/pro.3845.
Tingey M., Schnell S., Li Y., Junod S., Yu W., and Yang W.* (2020) Imaging Transmembrane Protein Transport across the Nuclear Envelope. A Closer Look at Membrane Proteins, Nova Science Publishers, edited by Simon G. Patching, pg 73-98.
2019
Tingey M., Mudumbi K., Schirmer E. and Yang W.* (2019) Casting a Wider Net: Differentiating between Inner Nuclear Envelope and Outer Nuclear Envelope Transmembrane Proteins. Int. J. Mol. Biol. 20(21). pii: E5248. doi: 10.3390/ijms20215248.
Liu X., Yao L., Yang W., Fei Y., Mi L. and Ma J. (2019) Spectroscopic fluorescent tracking of a single molecule in a live cell with a dual-objective fluorescent reflection microscope. Applied Physics Express 12, 112007.
Liu X., Wang B., Yao L., Fei Y., Mi L., Ma J and Yang W. (2019) 3D particle tracking using a dual-objective fluorescent reflection system with spherical aberration. J. Phys. D: Appl. Phys. 52, 385403.
Ruba A., Luo W. and Yang W.* (2019) The Ciliary Lumen Accommodates Passive Diffusion and Vesicle Trafficking in Cytoplasmic-Ciliary Transport. BioRxiv 704213; doi: https://doi.org/10.1101/704213.
Ruba A., Luo W., Kelich J., Tingey M. and Yang W.* (2019) 3D Microscopy Independent Approach for Obtaining 3D Super-resolution Information. J. Phys. Chem. B 123, 5107−5120.
Li. Y., Junod. S., Kelich, J., Yang, W.* (2019) Nuclear Export of RNA Molecules Studied by SPEED Microscopy. Methods. 153, 46-62
https://doi.org/10.1016/j.ymeth.2018.08.005.
2018
Ruba, A., Kelich J., Jiong, M., Yang, W.* (2018) Reply to “Deconstructing transport distribution reconstruction in the nuclear pore complex”. Nature Structural & Molecular Biology https://doi.org/10.1038/s41594-018-0162-1.
Yu J., Kelich J. and Yang W.* (2018) Assembly of Nuclear Pore Complex. NAMB Volume on Nuclear-Cytoplasmic Transport, Weidong Yang, Editor, Springer Publisher. Chapter 1, 1-14.
Li. Y., Junod. S., Kelich, J., Yang, W.* (2018) Nuclear Export of RNA Molecules Studied by SPEED Microscopy. Methods. https://doi.org/10.1016/j.ymeth.2018.08.005.
Li Y., Luo W. and Yang W. * (2018) Signal-Regulated Nuclear Transport and Accumulation of Smad Proteins studied by High-Speed Single-Molecule Microscopy. Biophysical J. 114, 2243. https://doi.org/10.1016/j.bpj.2018.03.018
Ruba A., Luo W. and Yang W.* (2018) Application of SPEED microscopy in primary cilia. J. Vis. Exp. (131), e56475, doi:10.3791/56475.
2017
Ruba A., Kelich J., Luo W., Yang W.* (2017) Obtaining 3D Super-resolution Information from 2D Super-resolution Images through a 2D-to-3D Transformation Algorithm. BioRxiv 188060; https://doi: https://doi.org/10.1101/188060.
Luo W., Ruba A., Lim R., Verhey K. and Yang W.* (2017) Axonemal Lumen Dominates Cytosolic Protein Diffusion inside Primary Cilium. Scientific Reports 7, 15793. Https://doi:10.1038/s41598-017-16103-z.
Mudumbi, K. C., & Yang, W.* (2017). Determination of membrane protein distribution on the nuclear envelope by single-point single-molecule FRAP. Current Protocols in Cell Biology, 76, 21.11.1–21.11.13. doi: 10.1002/cpcb.
Ma J.+, Kelich J.+, Junod S. and Yang W.* (2017) Super-resolution Mapping of Scaffold Nucleoporins in the Nuclear Pore Complex. Journal of Cell Science 130, 1299-1306 doi:10.1242/jcs.193912. + denotes equal contribution.
2016
Mudumbi K., Schirmer E. and Yang W.* (2016) Single-point FRAP distinguishes inner and outer nuclear membrane protein distribution. Nature Communications 7:12562, DOI:10.1038/ncomms12562.
Li C., Garyaynov A. and Yang W.* (2016) The selective barrier in the nuclear pore complex. Nucleus 7:430-446.
Ma J., Goryaynov A. and Yang W.* (2016) Super-resolution Three-dimensional Tomography of Interactions and Competitions in the Nuclear Pore Complex. Nature Structral & Molecular Biology 23:239-247.
Verhey K.* and Yang W. (2016) Permeability barriers for generating a unique ciliary protein and lipid composition. Current Opinion in Cell Biology 41, 109-116.
Ruba A. and Yang W.* (2016) O-GlcNAc-ylation in the Nuclear Pore Complex. Cellular and Molecular Bioengineering 9, 227-233.
Mudumbi K. and Yang W.* (2016) Probing protein distribution along the nuclear envelope in vivo by using single-molecule FRAP. Methods in Molecular Biology 1411, 113-122.
Ma J., Kelich J. and Yang W.* (2016) SPEED microscopy and its application in nucleocytoplasmic transport. Methods in Molecular Biology 1411, 503-518.
2015
Kelich J., Ma J., Dong B., Wang Q., Chin M., Magura C., Xiao W. and Yang W.* (2015) Super-resolution Imaging of Nuclear Import of Adeno-Associated Virus in Live Cells. Molecular Therapy – Methods & Clinical Development 2:15047. doi:10.1038/mtm.2015.47.
Mi L., Goryaynov A., Lindquist A., Rexach M. and Yang W.* (2015) Quantifying Nucleoporin Stoichiometry Inside Single Nuclear Pore Complexes In vivo. Scientific Reports 5:9312.
2014
Goryaynov A. and Yang W.* (2014) Role of molecular charge in nucleocytoplasmic transport. PLoS One. 9(2):e88792. doi: 10.1371/journal.pone.0088792.
Schnell S., Ma J. and Yang W.* (2014) Three-dimensional mapping of mRNA export through the nuclear pore complex. Genes, 5, 1032-1049.
Kelich J. and Yang W.* (2014) High-resolution imaging reveals new features of nuclear export of mRNA through the nuclear pore complex. Int. J. Mol. Sci. (Cover) 15, 14492-14504.
2013
Ma J., Liu Z., Michelotti N., Pitchiaya S., Veerapaneni R., Androsavich J., Walter N. and Yang W.* (2013) High-resolution three-dimensional mapping of mRNA export through the nuclear pore. Nature Communications, 4:2414 doi:10.1038/ncomms3414.
Yang W.* (2013) Distinct, but not completely separate spatial transport routes in the nuclear pore complex. Nucleus, 4,166-175.
2012
Ma J., Goryaynov A., Sarma A. and Yang W.* (2012) Self-Regulated Viscous Channel in the Nuclear Pore Complex. Proc. Natl. Acad Sci. USA, 109, 7326-7331.
Goryaynov A., Ma J. and Yang W.* (2012) Single-Molecule Studies of Nuclear Transport: from One Dimension to Three Dimensions. Integrative Biology (Cover) 4, 10-21.
2011
Mi L., Xiong R., Zhang Y., Li Z., Yang W., Chen J., Wang P.* (2011) Microscopic Observation of the Intercellular Transport of CdTe Quantum Dot Aggregates Through Tunneling-Nanotubes. JBNB, 2, 172-179.
Sarma A. and Yang W.* (2011) Calcium-Regulation of Nucleocytoplasmic Transport. Protein & Cell, 2, 291-302.
Yang W.* (2011) ‘’Natively Unfolded’’ Nucleoporins in Nucleocytoplasmic Transport: Clustered or Evenly Distributed? Nucleus, 2, 10-16.
2010
Ma J. and Yang W.* (2010) Three-Dimensional Distribution of Transient Interactions in the Nuclear Pore Obtained by Single-Molecule Snapshots. Proc. Natl. Acad. Sci. USA, 107, 7305-7310.
Goryaynov A., Sarma A, Ma J. and Yang W.* (2010) Single-Molecule Imaging of Nuclear Transport. J. Vis. Exp. (http://www.jove.com/index/Details.stp?ID=2040).
2008
Sun, C., Yang, W., Tu, L.-C. and Musser, S. M.* (2008) Single Molecule Measurements of Importin Alpha/Cargo Complex Dissociation at the Nuclear Pore. Proc. Natl. Acad. Sci. USA, 105, 8613-8618.
Selected Publications Before 2007
Yang W. and Musser M. S.* (2006) Nuclear transport time and efficiency are dependent on importin concentrations. Journal of Cell Biology, 174, 951-961.
Yang W. and Musser M. S. (2006) Visualizing single molecules transiting through nuclear pore complexes using narrow-field epifluorescence microscopy. Methods, 39, 316-328.
Wang P. N., Yang W., et al. (2004) Photoluminescence from High Purity InN and InGaN Nanoparticles Synthesized by Pulsed Discharge. Focus on Nanotechnology Research, Eugene V. Dirote (Editor), Nova Science Publisher. Chapter 8, 155-172.
Yang W., Gelles J. and Musser M. S. (2004) Imaging of single-molecule translocation through nuclear pore complexes. Proc. Natl. Acad. Sci. USA, 101, 12887-12892.
Yang W., Wang P.N., Li F.M. and Cheah K.W. (2002) Synthesis of oxygen-free InN nanoparticles by pulse discharge. Nanotechnology, 13, 65-68.