Mark Tingey
Research Interests: The Nuclear Envelope (NE) surrounding the nucleus is composed of an inner nuclear membrane (INM) and outer nuclear membrane (ONM). The Nuclear Pore Complex (NPC) provides a pathway through which macromolecules and proteins may pass from the ONM to the INM or vis versa. This passage takes place through either the central channel or peripheral channel of the NPC. My focus is on the Nuclear Envelope Transmempraine proteins (NETs) which stud the Endoplasmic Reticulum (ER), ONM and INM. Particlularly, I am interested in the method by which these proteins translocate from the ONM to INM.
The mechanism of NETs transport across the NE remains a topic of discussion among researchers. To interrogate this question, our lab makes use of single-molecule microscopy techniques to directly image and measure the pathway, dynamics, and direction of protein transport across the NE.
Publications
Dixon, C. R.,
Malik, P., Jose, I., Saiz-Ros, N., de Lima Alves, F., Tingey, M., Gaunt, E.,
Richardson, A. C., Kelly, D. A., & Goldberg, M. W. (2021). STING nuclear
partners contribute to innate immune signaling responses. Iscience, 24(9), 103055.
Junod, S. L.,
Tingey, M., Rush, C., Alkurdi, A., Bajoria, K., & Yang, W. (2023).
Obtaining 3D super-resolution images by utilizing rotationally symmetric
structures and 2D-to-3D transformation. Computational
and Structural Biotechnology Journal.
Li, Y., Aksenova,
V., Tingey, M., Yu, J., Ma, P., Arnaoutov, A., Chen, S., Dasso, M., & Yang,
W. (2021). Distinct roles of nuclear basket proteins in directing the passage
of mRNA through the nuclear pore. Proceedings
of the National Academy of Sciences,
118(37), e2015621118.
Li, Y., Tingey,
M., Ruba, A., & Yang, W. (2020). High-speed super-resolution imaging of
rotationally symmetric structures using SPEED microscopy and 2D-to-3D
transformation. Nature protocols.
Ruba, A., Luo,
W., Kelich, J., Tingey, M., & Yang, W. (2019). 3D tracking-free approach
for obtaining 3D super-resolution information in rotationally symmetric
biostructures. The Journal of Physical
Chemistry B, 123(24), 5107-5120.
Schnell, S. J., Tingey, M., & Yang, W. (2022). Speed Microscopy: High-Speed Single Molecule Tracking and Mapping of Nucleocytoplasmic Transport. In The Nuclear Pore Complex: Methods and Protocols (pp. 353-371). Springer US New York, NY.
Tingey, M., Li,
Y., & Yang, W. (2021). Protocol for single-molecule fluorescence recovery
after photobleaching microscopy to analyze the dynamics and spatial locations
of nuclear transmembrane proteins in live cells. STAR protocols, 2(2),
100490.
Tingey, M., Li,
Y., & Yang, W. (2022). Selective Degradation and Quantification of
Nucleoporins in the Nuclear Pore by Auxin‐Inducible Degrons and Single‐Molecule
Microscopy. Current Protocols, 2(9), e520.
Tingey, M., Li,
Y., Yu, W., Young, A., & Yang, W. (2022). Spelling out the roles of
individual nucleoporins in nuclear export of mRNA. Nucleus, 13(1), 172-195.
Tingey, M.,
Mudumbi, K. C., Schirmer, E. C., & Yang, W. (2019). Casting a Wider Net:
Differentiating between Inner Nuclear Envelope and Outer Nuclear Envelope
Transmembrane Proteins. International
journal of molecular sciences, 20(21),
5248.
Tingey, M., Ruba,
A., & Yang, W. (2023). High-SPEED super-resolution SPEED microscopy to
study primary cilium signaling in vivo.
Tingey, M.,
Schnell, S., Yu, W., Saredy, J., Junod, S., Patel, D., Alkurdi, A., & Yang,
W. (2022). Technologies Enabling Single-Molecule Super-Resolution Imaging of
mRNA. Cells 2022, 11, 3079.
Tingey, M.,
Schnell, S. J., Li, Y., Junod, S., Yu, W., & Yang, W. (2020). IMAGING
TRANSMEMBRANE PROTEIN TRANSPORT ACROSS THE NUCLEAR ENVELOPE. In A CLOSER LOOK AT MEMBRANE PROTEINS (pp.
351). INDEPENDENT PUBLISHING NETWORK.
Tingey, M.,
Schnell, S. J., Yu, W., Saredy, J., Junod, S., Patel, D., Alkurdi, A. A., &
Yang, W. (2022). Technologies Enabling Single-Molecule Super-Resolution Imaging
of mRNA. Cells, 11(19), 3079.
Tingey, M., &
Yang, W. (2022). Unraveling docking and initiation of mRNA export through the
nuclear pore complex. BioEssays, 44(8), 2200027.