Andrew Ruba
Research interests: Primary cilia are signaling organelles projecting from the side of eukaryotic cells. They are involved in a variety of signal transduction pathways ranging from planar cell polarity, Hedgehog signaling, and neuronal signaling to nutrient sensing, mechanosensation, olfaction, phototransduction, and cellular growth.
Since no protein synthesis occurs in primary cilia, all protein involved in these processes are imported through a selectivity barrier at the transition zone at the base of primary cilia. My interest is to use single molecule tracking and super-resolution microscopy to determine the 3D transport routes and mechanisms that these proteins use to enter primary cilia.
Publications
Li, Y., Junod, S.L., Ruba, A., Kelich, J.M., Yang, W. (2019). Nuclear export of mRNA molecules studied by SPEED microscopy. Methods.
Ruba, A., Kelich, J.M., Ma, J., Yang, W. (2018). Reply to ‘Deconstructing transport-distribution reconstruction in the nuclear-pore complex’. Nature Structural & Molecular Biology.
Ruba, A., Luo, W., Yang, W. (2017). Application of high-speed super-resolution SPEED microscopy in live primary cilium. Journal of Visualized Experiments.
Luo, W., Ruba, A., Takao, D., Zweifel, L., Lim, R., Verhey, K., Yang, W. (2017). A New Protein Transport Pathway inside the Primary Cilium Revealed by High-Speed Super-Resolution Microscopy. Scientific Reports.
Ruba, A., Yang, W. (2016). O-GlcNAc-ylation in the Nuclear Pore Complex. Cellular and Molecular Bioengineering.