{"id":635,"date":"2019-01-17T20:04:26","date_gmt":"2019-01-17T20:04:26","guid":{"rendered":"http:\/\/yanglab.com\/?page_id=635"},"modified":"2025-07-24T21:39:36","modified_gmt":"2025-07-24T21:39:36","slug":"mark-tingey","status":"publish","type":"page","link":"http:\/\/yanglab.com\/index.php\/home\/lab-personnel\/mark-tingey\/","title":{"rendered":"Mark Tingey"},"content":{"rendered":"\t\t
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Mark Tingey<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Tingey\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Research Interests:\u00a0 <\/b>The Nuclear Envelope (NE) surrounding the nucleus is composed of an inner nuclear membrane (INM) and outer nuclear membrane (ONM). The Nuclear Pore Complex (NPC) provides a pathway through which macromolecules and proteins may pass from the ONM to the INM or vis versa. This passage takes place through either the central channel or peripheral channel of the NPC. My focus is on the Nuclear Envelope Transmempraine proteins (NETs) which stud the Endoplasmic Reticulum (ER), ONM and INM. Particlularly, I am interested in the method by which these proteins translocate from the ONM to INM.<\/p>

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The mechanism of NETs transport across the NE remains a topic of discussion among researchers. To interrogate this question, our lab makes use of single-molecule microscopy techniques to directly image and measure the pathway, dynamics, and direction of protein transport across the NE.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Publications<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Dixon, C. R.,\nMalik, P., Jose, I., Saiz-Ros, N., de Lima Alves, F., Tingey, M.<\/b>, Gaunt, E.,\nRichardson, A. C., Kelly, D. A., & Goldberg, M. W. (2021). STING nuclear\npartners contribute to innate immune signaling responses. Iscience<\/b><\/i>, 24<\/i>(9), 103055. <\/o:p><\/p>

Junod, S. L.,\nTingey, M.<\/b>, Rush, C., Alkurdi, A., Bajoria, K., & Yang, W. (2023).\nObtaining 3D super-resolution images by utilizing rotationally symmetric\nstructures and 2D-to-3D transformation. Computational\nand Structural Biotechnology Journal<\/b><\/i>. <\/o:p><\/p>

Li, Y., Aksenova,\nV., Tingey, M.<\/b>, Yu, J., Ma, P., Arnaoutov, A., Chen, S., Dasso, M., & Yang,\nW. (2021). Distinct roles of nuclear basket proteins in directing the passage\nof mRNA through the nuclear pore. Proceedings\nof the National Academy of Sciences<\/b><\/i>,\n118<\/i>(37), e2015621118. <\/o:p><\/p>

Li, Y., Tingey,\nM.<\/b>, Ruba, A., & Yang, W. (2020). High-speed super-resolution imaging of\nrotationally symmetric structures using SPEED microscopy and 2D-to-3D\ntransformation. Nature protocols<\/b><\/i>. <\/o:p><\/p>

Ruba, A., Luo,\nW., Kelich, J., Tingey, M.<\/b>, & Yang, W. (2019). 3D tracking-free approach\nfor obtaining 3D super-resolution information in rotationally symmetric\nbiostructures. The Journal of Physical\nChemistry B<\/i>, 123<\/i>(24), 5107-5120. <\/o:p><\/p>

Schnell, S. J.,\nTingey, M.<\/b>, & Yang, W. (2022). Speed Microscopy: High-Speed Single Molecule\nTracking and Mapping of Nucleocytoplasmic Transport. In The Nuclear Pore Complex: Methods and Protocols<\/b><\/i> (pp. 353-371).\nSpringer US New York, NY.<\/p>

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Tingey, M.<\/b>, Li,\nY., & Yang, W. (2021). Protocol for single-molecule fluorescence recovery\nafter photobleaching microscopy to analyze the dynamics and spatial locations\nof nuclear transmembrane proteins in live cells. STAR protocols<\/i>, 2<\/i>(2),\n100490. <\/o:p><\/p>

Tingey, M.<\/b>, Li,\nY., & Yang, W. (2022). Selective Degradation and Quantification of\nNucleoporins in the Nuclear Pore by Auxin\u2010Inducible Degrons and Single\u2010Molecule\nMicroscopy. Current Protocols<\/b><\/i>, 2<\/i>(9), e520. <\/o:p><\/p>

Tingey, M.<\/b>, Li,\nY., Yu, W., Young, A., & Yang, W. (2022). Spelling out the roles of\nindividual nucleoporins in nuclear export of mRNA. Nucleus<\/b><\/i>, 13<\/i>(1), 172-195. <\/o:p><\/p>

Tingey, M.<\/b>,\nMudumbi, K. C., Schirmer, E. C., & Yang, W. (2019). Casting a Wider Net:\nDifferentiating between Inner Nuclear Envelope and Outer Nuclear Envelope\nTransmembrane Proteins. International\njournal of molecular sciences<\/b><\/i>, 20<\/i>(21),\n5248. <\/o:p><\/p>

Tingey, M.<\/b>, Ruba,\nA., & Yang, W. (2023). High-SPEED super-resolution SPEED microscopy to\nstudy primary cilium signaling in vivo. <\/o:p><\/p>

Tingey, M.<\/b>,\nSchnell, S., Yu, W., Saredy, J., Junod, S., Patel, D., Alkurdi, A., & Yang,\nW. (2022). Technologies Enabling Single-Molecule Super-Resolution Imaging of\nmRNA. Cells <\/b>2022, 11, 3079. <\/o:p><\/p>

Tingey, M.<\/b>,\nSchnell, S. J., Li, Y., Junod, S., Yu, W., & Yang, W. (2020). IMAGING\nTRANSMEMBRANE PROTEIN TRANSPORT ACROSS THE NUCLEAR ENVELOPE. In A CLOSER LOOK AT MEMBRANE PROTEINS<\/i> (pp.\n351). INDEPENDENT PUBLISHING NETWORK. <\/o:p><\/p>

Tingey, M.<\/b>,\nSchnell, S. J., Yu, W., Saredy, J., Junod, S., Patel, D., Alkurdi, A. A., &\nYang, W. (2022). Technologies Enabling Single-Molecule Super-Resolution Imaging\nof mRNA. Cells<\/b><\/i>, 11<\/i>(19), 3079. <\/o:p><\/p>

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Tingey, M.<\/b>, &\nYang, W. (2022). Unraveling docking and initiation of mRNA export through the\nnuclear pore complex. BioEssays<\/i>,<\/b> 44<\/i>(8), 2200027. <\/o:p><\/p>\n

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\n\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\tEnvelope<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/i>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\tLink<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/i>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\tLinkedin<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/i>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\tTwitter<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/i>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Mark Tingey Research Interests:\u00a0 The Nuclear Envelope (NE) surrounding the nucleus is composed of an inner nuclear membrane (INM) and outer nuclear membrane (ONM). The Nuclear Pore Complex (NPC) provides a pathway through which macromolecules and proteins may pass from the ONM to the INM or vis versa. This passage […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":105,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/template-pagebuilder-full-width.php","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-635","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PaD2aX-af","_links":{"self":[{"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/pages\/635","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/comments?post=635"}],"version-history":[{"count":16,"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/pages\/635\/revisions"}],"predecessor-version":[{"id":1103,"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/pages\/635\/revisions\/1103"}],"up":[{"embeddable":true,"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/pages\/105"}],"wp:attachment":[{"href":"http:\/\/yanglab.com\/index.php\/wp-json\/wp\/v2\/media?parent=635"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}